【报告题目】Spatial Light Interference Microscopy (SLIM) for quantitative biomedicine
【报 告 人】Prof. Gabriel Popescu, University of Illinois at Urbana-Champaign, Urbana
Spatial Light Interference Microscopy (SLIM)
for quantitative biomedicine
Prof. Gabriel Popescu
Quantitative Light Imaging Laboratory
Department of Electrical and Computer Engineering
Beckman Institute for Advanced Science & Technology
University of Illinois at Urbana-Champaign, Urbana, IL 61801
Research Seminar: 2 hours
Most living cells do not absorb or scatter light significantly, i.e. they are essentially transparent, or phase objects. Phase contrast microscopy proposed by Zernike in the 1930’s represents a major advance in intrinsic contrast imaging, as it reveals inner details of transparent structures without staining or tagging. While phase contrast is sensitive to minute optical path-length changes in the cell, down to the nanoscale, the information retrieved is only qualitative. Quantifying cell-induced shifts in the optical path-lengths permits nanometer scale measurements of structures and motions in a non-contact, non-invasive manner. Thus, quantitative phase imaging (QPI) has recently become an active field of study and various experimental approaches have been proposed.
We have developed Spatial Light Interference microscopy (SLIM) as a highly sensitive QPI method. Due to its nanometer pathlength sensitivity, SLIM enables interesting structure and dynamics studies over broad spatial (nanometers-centimeters) and temporal (milliseconds-weeks) scales. I will review our recent results on applying SLIM to basic cell studies, such as intracellular transport, cell growth, and single cell tomography. Recently, we have demonstrated that SLIM is a valuable tool for cancer diagnosis and prognosis in unlabeled biopsies. This capability is particularly valuable in prostate pathology.
abriel Popescu is an Associate Professor in Electrical and Computer Engineering, University of Illinois at Urbana-Champaign. He received his Ph.D. in Optics in 2002 from the School of Optics/ CREOL (now the College of Optics and Photonics), University of Central Florida. Dr. Popescu continued his training with Michael Feld at M.I.T., working as a postdoctoral associate. He joined Illinois in August 2007 where he directs the Quantitative Light Imaging Laboratory (QLI Lab) at the Beckman Institute for Advanced Science and Technology. Dr. Popescu has served as Associate Editor of Optics Express and Biomedical Optics Express, Editorial Board Member for Journal of Biomedical Optics and Scientific Reports. He has authored a book, edited another book, authored 140 journal publications, 200 conference presentations, 32 patents, gave 170 plenary/invited/seminar talks. Dr. Popescu founded Phi Optics, Inc., a start-up company that commercializes quantitative phase imaging technology. He is OSA Fellow and SPIE Fellow.